How do I calculate my peptide dose?

To calculate your peptide dose, input the amount of peptide in your vial (in mg or IU), the volume of bacteriostatic water used for reconstitution (in mL), and your desired dose. The calculator will determine the exact volume to inject and show you where to draw the liquid on a standard insulin syringe.

How many units do I draw for 250mcg of BPC-157 in 3mL water?

For a 10mg BPC-157 vial reconstituted with 3mL bacteriostatic water, a 250mcg (0.25mg) dose requires drawing to the 7.5-unit mark on a 100-unit syringe (0.075 mL). Use PeptideGraph’s Dose Calculator to confirm for any vial size or dose.

How many doses can I get from a 5mg BPC-157 vial at 250mcg per dose?

A 5mg vial at 250mcg (0.25mg) per dose gives 20 doses. PeptideGraph shows the doses-per-vial count automatically as you enter your inputs.

What is peptide reconstitution?

Peptide reconstitution is the process of mixing a lyophilized (freeze-dried) peptide powder with a sterile solvent, usually bacteriostatic water, to create an injectable solution.

How do I read an insulin syringe for peptides?

Insulin syringes are measured in units (IU). A standard 100-unit syringe holds 1.0 mL of liquid, meaning 10 units equal 0.1 mL. Our calculator provides a visual guide for 30-unit, 50-unit, and 100-unit syringes to help you draw the exact amount.

What is the correct BAC water calculator method for peptides?

A BAC (bacteriostatic) water calculation factors in your total peptide amount and your added BAC water to compute the concentration. Once you know the concentration, you can easily determine how many units to draw for your specific dose. Our calculator does all this math for you instantly.

Where can I find peptide mixing instructions?

To mix your peptides, typically you inject bacteriostatic water directly into the lyophilized powder vial, allowing it to dissolve gently without shaking. Our dose calculator helps you safely identify your precise injection volume based on that specific reconstitution mix.

Peptide Dose Calculator — Syringe Units & Injection Volume

Peptide in Vial

Bacteriostatic Water Added

Desired Dose

Your Syringe

Reconstituted Concentration

3.33 mg/mL

Doses per Vial

20doses

Volume to Inject

0.150mL

Units on Syringe

15.0units

100 unit syringe

Draw to the 15.0 unit mark to deliver 0.5 mg

How to Use the Peptide Dose Calculator

Calculating the correct peptide dosage can be confusing, especially when converting between milligrams (mg), micrograms (mcg), and insulin syringe units (IU). Our Peptide Dose Calculator simplifies this process. Simply input the amount of peptide in your vial, the volume of bacteriostatic water used for reconstitution, and your desired dose. The calculator will instantly determine the exact volume to inject and visually show you where to draw the liquid on a standard insulin syringe.

What is Reconstitution?

Peptides like BPC-157, TB-500, and Semaglutide typically arrive in a lyophilized (freeze-dried) powder form. Before they can be used, they must be reconstituted—mixed with a sterile solvent, usually bacteriostatic water. The amount of water you add determines the concentration of the solution, which directly impacts how many units you need to draw into your syringe for a specific dose.

Reading an Insulin Syringe

Insulin syringes are measured in units (IU) or milliliters (mL). A standard 100-unit syringe holds exactly 1.0 mL of liquid. Therefore, 10 units equal 0.1 mL. Our calculator supports 30-unit (0.3 mL), 50-unit (0.5 mL), and 100-unit (1.0 mL) syringes, providing a visual guide to ensure you draw the exact amount required for your research.

Common Dosing Questions

How many units do I draw for 250mcg BPC-157 reconstituted with 3mL of water?

A 10mg BPC-157 vial in 3mL of bacteriostatic water has a concentration of 3.33 mg/mL. A 250mcg (0.25mg) dose requires 0.075 mL—draw to the 7.5-unit mark on a 100-unit syringe. This vial yields 40 doses at 250mcg each.

How many units do I inject for 0.5mg Semaglutide from a 10mg vial in 3mL water?

Concentration: 3.33 mg/mL. Volume: 0.5 ÷ 3.33 = 0.15 mL = 15 units on a 100-unit syringe. At 0.5mg/week, that’s 20 doses per vial.

How do I convert mcg to mL for a peptide injection?

Divide the vial amount in mg by the water volume in mL to get concentration (mg/mL). Then divide your dose in mg by the concentration to get volume in mL. Multiply by 100 for syringe units. For example: 10mg ÷ 2mL = 5mg/mL; 500mcg (0.5mg) ÷ 5mg/mL = 0.1mL = 10 units.

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